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pegfp n1 htrim32  (Addgene inc)


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    Structured Review

    Addgene inc pegfp n1 htrim32
    Pegfp N1 Htrim32, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+htrim32/pEGFP-N1_hTRIM32+(Plasmid+%2369541)/bio_rxiv__64898__2026__02__11__705390-206-23-24
    Average 93 stars, based on 6 article reviews
    pegfp n1 htrim32 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: CSNK2 suppresses autophagy by activating FLN-NHL-containing TRIM proteins
    Article Snippet: The insert was amplified by PCR using the Phusion High-Fidelity PCR Kit (NEB, E0553L) according to the manufacturer’s instructions with the primers T71f and T71r purchased from Biomers. .. The pIRES_FLAG vector was linearized with NotI and MluI restriction enzymes. pEGFP-N1_hTRIM32 was a gift from Martin Dorf (Addgene, 6954147). .. The ORF of TRIM32 was subcloned into the pIRES_FLAG vector using NEBuilder HiFi DNA Assembly (NEB, E2621X) according to the manufacturer’s instructions.

    Expressing:

    Article Title: TRIM32–UBQLN2–p62 axis promotes TDP-43 inclusion formation and amyloid aggregation through shuttle condensates
    Article Snippet: .. UBQLN2 STI1-I domain (161-297) was subcloned into vector pGEX-6P-1 (GE Healthcare) between the EcoRI and XhoI restriction sites for expression in E. coli. pEGFP-N1_hTRIM32 (Addgene: # 69541 ), HA-p62 (Addgene: # 28027 ), mCherry-p62 (Addgene: # 187901 ), mCherry-ANXA11 ( Addgene: # 164203 ), pcDNA3.2 TDP-43 YFP ( Addgene: # 84911), pcDNA3.2 TDP-43 NLS1 YFP ( Addgene: # 84912), were purchased from Addgene. pEGFP-N1_hTRIM32 (Addgene: #69541) and UBQLN2-mCherry (from Dr. Carlos Castañeda) were used as the backbone for mCardinal-TRIM32 and UBQLN2-mCardinal, respectively. ..



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